I was curious if anyone had heard of any effort or studies for CFS/ME that utilized Next Generation Metagenomic Shotgun Sequencing of Blood or Plasma for in hunting for underlying infections that might be causing an individual's CFS/ME symptoms? I know there has been many attempts to find any underlying infections in CFS/ME and I'm sure many individual's doctors have run many of the usual pathogen antibody or PCR tests to look for an underlying infection. Until recently,a doctor or ID doctor had to make a guess as to a likely pathogen and run one test per guess or in some cases use a fixed pathogen panel to cover the 10 guesses made by the lab who developed the panel. A panels example might be the Luminex GI Pathogen Panel. https://www.luminexcorp.com/gastrointestinal-pathogen-panel/ White Paper : https://www.luminexcorp.com/?wpdmdl=13681 If someone is in the intensive care unit with a life threatening suspected infection, the doctor(s) throw multiple antimicrobials at the patient and guess as to what to test for. If they are wrong, the patient might die. Recently both UCSF and a company Karius Dx have been offering NGS sequencing of blood or Serum to critically ill patients in hospitals and are able to detect free DNA of anything in their body. They report levels that are above background noise that "might" be an infection with astounding success. Their testing is both state of the art and held in high regard in both the scientific and medical communities but the ID doctors to date have convinced them to restrict the offering to hospital patients with serious suspected infections and not allowing testing of chronic conditions such as CFS/ME. They describe their test as: A quantitative next-generation sequencing test to help clinicians rapidly diagnose infectious diseases. Our analytically validated assay identifies microbial cell-free DNA in plasma from bacteria, DNA viruses, fungi, molds and protozoa. You can read about Karius Dx here: https://kariusdx.com/products/karius-test This is there currently validated list of pathogens they detect: https://kariusdx.com/pathogenlist/3.4 UCSF has no less than 3 labs pursuing the same approach: https://nextgendiagnostics.ucsf.edu/our-research/ The cost of the Karius Dx test to the hospital is $2000 but this would the cost of about 3-4 wrong guesses for PCR pathogen tests or similar to a panel mentioned earlier. The beauty is that the doctor does not need to make any guesses based on symptoms or history and can simply screen for anything leaving free DNA in the bloodstream. An infection most anywhere in your body will "leak" broken down free DNA into your blood as the pathogen complete its life cycle, dies and enters the bloodstream for removal. The test will detect any microbial DNA so the doctor must be able to look at the report and eliminate things like reactivated Herpes. Its a shame that the ID doctors are controlling how Karius Dx offers its test. It seems obvious to me that this would be a no-brainer study for CFS/ME. I would consider funding such a study under the right circumstances. I suspect Karius will eventually open this testing up to chronic conditions but didn't want to rock the boat for now. That just pushes patients and doctors to use less reputable labs with no validation. I notice the UCSF labs also require permission by UCSF ID so appear to have the same restrictions.