The 4I Hypothesis: A Neuro-Immunological Explanation for Characteristic Symptoms of ME

J William M Tweedie

Well-Known Member
Frank NM Twisk, ME-de-patiënten Foundation, Zonnedauw 15, 1906 HB, Limmen, The Netherlands
ABSTRACT
Characteristic symptoms of Myalgic Encephalomyelitis (ME)
are (muscle) weakness, muscle pain, cognitive deficits, neurological abnormalities, but above all post-exertional malaise: a long-lasting increase of symptoms after a minor exertion. In contrast, Chronic Fatigue Syndrome (CFS) is primarily defined by chronic fatigue. Since chronic fatigue is not mandatory for the diagnosis ME, and post-exertional malaise and cognitive deficits are not obligatory for the diagnosis CFS, the case criteria for ME and CFS define two distinct, partly overlapping nosological entities. ME and CFS are considered to be enigmatic diseases, qualified by some authors as medically unexplained syndromes of functional syndromes. However, specific abnormalities consistently observed over the years and their direct and indirect sequels can plausibly explain characteristic symptoms, e.g. exhaustion and pain. Abnormalities established repetitively incorporate immunological aberrations (inflammation, immune activation, immunosuppression, and immune dysfunction), persistent and/or reactivating infections, gastro-intestinal dysbiosis, oxidative and nitrosative stress, mitochondrial dysfunction, a (prolonged) deviant response to exertion and orthostatic stress, circulatory deficits, and neurological abnormalities.This article depicts the 4I hypothesis, an explanatory model for ME (CFS) with a central role for four types of immunological abnormalities: inflammation, (Th2-predominated) immune activation, immunosuppression, and immune dysfunction. The potential direct sequels of these abnormalities, e.g. increased oxidative and nitrosative stress, (reactivating or chronic) infections, and their possible indirect consequences, e.g. mitochondrial dysfunction, hypothalamic-pituitary-adrenal axis (HPA) axis hypofunction, and cardiovascular dysregulation, can plausibly explain various distinctive symptoms of ME/CFS, e.g. exhaustion, (muscle) weakness, pain, cognitive deficits, a flu-like feeling, and post-exertional malaise.

International Journal of Neurology Research 2015; 1(2): 20-38 Available from: URL: http://www.ghrnet.org/index.php/ijnr/article/view/1058

CORE TIPS
ME and CFS are two distinct, partially overlapping diagnostic entities.
More consistent findings in ME and/or CFS relate to four types of immunological abnormalities: inflammation (I1), (Th2-dominated) immune activation (I2), immunosuppression (I3), and immune dysfunction (I4).

The potential sequels of these abnormalities encompass oxidative/nitrosative stress and infections.
The possible indirect consequences of the immunological aberrations and increased oxidative/nitrosative stress include neurological abnormalities, mitochondrial dysfunction and cardiovascular disturbances.
The direct and indirect sequels of the immunological abnormalities can plausibly explain distinctive symptoms of ME/CFS, e.g. cognitive deficits, and post-exertional malaise: a prolonged aggravation of characteristic symptoms, e.g. cognitive impairments and pain, after a minor exertion.

INTRODUCTION
Although ME and CFS are often used interchangeably, the case criteria for ME[1] and CFS[2] define two distinct, partially overlapping diagnostic entities[3]. The diagnosis ME requires specific neurological/neurocognitive and immunological symptoms and energy production and/or transport impairment, but the distinctive feature of ME is post-exertional malaise or neuro-immune exhaustion: a pathological inability to produce sufficient energy on demand resulting into symptom exacerbation, e.g. flu-like symptoms and pain, after minor exertion[1]. The distinctive feature of CFS[2] on the other hand is (unexplained) chronic fatigue, which should be accompanied by at least four out of a eight symptoms, e.g. sore throat, unrefreshing sleep, and headaches. While post-exertional malaise is not obligatory for CFS[2], fatigue is not mandatory for the diagnosis ME[1]. The distinction between patients with post-exertional malaise and without post-exertional malaise seems to be reflected by specific immunological differences[4,5]. Although ME and CFS criteria select partially overlapping, partially disjoint patient groups, the majority of the research into ME/CFS in the last decades has been conducted in patients selected by CFS criteria[2]. However, since many optional symptoms of CFS are mandatory for the diagnosis ME, the CFS criteria also apply to a substantial ME patient subgroup reporting fatigue (Figure 1). In conclusion, while ME is a neuro-immunological disease in nature[1], the CFS criteria[2] select a heterogeneous patient population of people with self-reported chronic fatigue.

SYMPTOMS
Notwithstanding the debate about the distinction between ME and CFS[6-8] and definitional criteria of ME and CFS[9], including obligatory symptoms, many patients with ME/CFS experience a plethora of symptoms[3], which differ inter-individually and seem to fluctuate in number and severity within an individual over time as a consequence of daily activity[10]. Symptoms experienced by substantial patient subgroups are: post-exertional malaise, fatigue/lack of energy, muscle weakness, (muscle/joint) pain, cognitive impairment (brain fog), a flu-like feeling, sleep dysfunction (unrefreshing sleep), hypersensitivity to food, light, sound and odours (central sensitisation), stress intolerance, orthostatic intolerance and depression[9] (Table 1). Various characteristic symptoms can be assessed objectively using well-accepted methods[3], e.g. neurocognitive tests, while other symptoms due to their nature, e.g. (muscle) pain, cannot be assessed objectively.

ABNORMALITIES
Partly due to the heterogeneity[32] of the CFS[2] patient population and the variety of methods employed and samples investigated, research into ME/CFS has yielded contradictory results. However, various typical aberrations (Table 2) have been observed repetitively in the ME/CFS patient population or subgroups thereof[1,33], Several abnormalities are confirmed by differential gene expression[34-37].

Onset
Contrary to gradual onset ME/CFS, sudden-onset ME/CFS is often preceded by a (viral) infection/flu-like illness[94,95]. The onset is reflected by distinctive immunological aberrations[96,97] and other abnormalities[98,99]. Several pathogens have been reported to initiate ME/CFS, e.g. Epstein-Barr virus[100], parvovirus B19[101], and enteroviruses[48]. For example, 10-15% of individuals do not recover from infectious mononucleosis and fulfil the criteria for CFS[2] after six months[100,102]. The severity of the acute infection seems to predict the clinical outcome, rather than demographic, psychological, or microbiological factors[102,103].

Infections
Although contradicted by some studies, applying various methods various studies have found (multiple) infections or related antigens in patient subgroups (Table 3).
Intestinal dysbiosis and hyperpermeability
Immunological abnormalities
Increased oxidative and nitrosative stress

Elevated oxidative and nitrosative stress[36,88], increased levels of superoxide (O2-), nitric oxide (NO) and peroxynitrite (ONOO-), oxidative and nitrosative damage to DNA, proteins, lipids etc.[147-149], and antioxidant depletion / increased antioxidant activity, e.g. vitamin A[150], B[151], C[56], D[152], E[56], glutathione[153], super oxide dismutase[62] and zinc[154], have been observed repetitively.

Mitochondrial dysfunction and damage
Some studies have found structural mitochondrial damage, e.g. branching and fusion of mitochondrial cristae /mitochondrial degeneration[63], substantially higher rates of deletion of common 4977 bp of mitochondrial DNA[155] and unusual patterns of mitochondrial DNA deletions in skeletal muscle[59], while other studies implicate mitochondrial dysfunction[60,61,156]. Future research should provide clarity whether hypometabolism[157] and low oxygen uptake[75,78] and extraction[158] in ME/CFS is due to mitochondrial dysfunction and/or mitochondrial damage, circulatory deficits (see next paragraph) or other causes.

Low blood volume, cardiac output and/or blood and oxygen supply
Orthostatic abnormalities
Neurological abnormalities
HPA axis dysfunction
Abnormal responses to exercise


PLAUSIBLE CAUSAL RELATIONSHIPS BETWEEN ABNORMALITIES
Figure 2, the 4I hypothesis: a key role for four types of immunological abnormalities
Four immunological abnormalities underpin the 4I hypothesis for ME/CFS (Figure 2): inflammation (I1), (Th2-predominant) immune activation (I2), immunosuppression (I3) and immune dysfunction (I4).

Potential immunological stimuli in ME/CFS
The immune system seems to face three potential stimuli in ME/CFS: pathogens and associated antigens, due to acute, chronic and/or reactivated infections (Figure 2, A, B1 and B2), gastro-intestinal inflammation and hyperpermeability of the intestines, possibly resulting into translocation of enterobacteria to the blood stream (Figure 2, D), and auto-epitopes, due to oxidative and nitrosative damage to proteins, lipids etc. (Figure 3, K).

ME/CFS is often precipitated by infections, the severity of which seems to predict the clinical outcome[102,103]. Some studies implicate persistency of the pathogens[48] or antigens related to the pathogen[190] that instigated ME/CFS. Various infectious agents linked to ME/CFS are able to produce a persistent active infection, thereby establishing a constant incitement to the immune system[45]. In addition, some pathogens associated with ME/CFS, e.g. EBV[191] and HHV-6A/B[192], are known to induce a life-long latent infection. Several studies suggest reactivation of these pathogens[111,193], possible due to immunosuppression and immune dysfunction, e.g. a deficient EBV-specific B- and T-cell response[191]. The chronic or reactivated infection-hypothesis is contested[194]. However, as summarized, many studies have observed active infections in substantial patient subgroups. Infections would explain inflammation and immune activation in ME/CFS (Figure 2, A). It is also known that certain pathogens evade the immune system by immune modulation (Figure 2, B1 and B2), e.g. inhibiting the innate immune response, disrupting of T-cell function, and inducing a Th1->Th2 switch[195,196].

Another possible immunological challenge relates to intestinal dysbiosis[49,51] and inflammation[130] and increased permeability of the intestinal barrier, thereby allowing enterobacteria to enter the blood stream, as indicated by elevated serum IgA levels against lipopolysaccharides (LPS) of gram-negative enterobacteria[50]. Intestinal dysbiosis and inflammation[54] and increased IgA responses to the LPS of commensal bacteria[53] have been associated with systemic inflammation and immune activation (Figure 2, D). It is known the gastro-intestinal tract can modulate the central nervous system by various blood-brain-barrier mediated mechanisms, e.g. through the secretion of NO and cytokines, thereby influencing behaviour[197].

Finally, auto-epitopes, originating from oxidative and nitrosative damage to proteins and lipids (Figure 3, J), can induce autoimmune responses[198,199] (Figure 3, K). Although insufficiently explained, elevated levels of auto-antibodies have frequently been observed in ME/CFS, e.g. against serotonin[200,201], gangliosides[200], phospholipids[200], including mitochondrial cardiolipins[202,203], antinuclear antibodies[47,204], muscarinic cholinergic receptor[204] and ssDNA[205].

A vicious cycle of oxidative and nitrosative stress induced by inflammation
Oxidative and nitrosative stress play an important intermediate role in the 4I hypothesis. Inflammation will generate oxidative and nitrosative stress[206,207] (Figure 3, E), while oxidative and nitrosative stress can induce or amplify inflammation[208,209] (Figure 3, F). NO and reactive oxygen species (ROS) exert multiple immune-modulating effects[210,211]. ROS and reactive nitrogen species (RNS), including NO, can suppress NK[212,213] and T[214,215] cell cytotoxicity, which could contribute to immunosuppression (Figure 3, G). High levels of NO, produced by cytotoxic activated macrophages[216] may also play an important role in the shift from a Th1 to Th2 response[217,218] (Figure 3, H). While NO seems to suppress both Th1- and Th2-cell-mediated immunity at the early proliferation stage, high levels of NO appear to inhibit the Th1-cell differentiation of mature T helper (Th) cells[219]. Through several feedback loops oxidative and nitrosative stress can induce a self-perpetuating cycle of elevated oxidative and nitrosative stress, inflammation, elevated N-methyl-D-aspartate (NMDA) receptor activity, and ATP depletion, which is mediated by O2-, NO and ONOO-[220] (Figure 3, I).

HPA axis hypofunction as a potential sequel of inflammation, immune activation and oxidative stress
The HPA axis and the immune system are bidirectionally interconnected[221]. Some authors have suggested that the immunological abnormalities in ME/CFS, e.g. inflammation, are secondary to HPA axis hypofunction[177], possibly due to a stress crash: a switch from HPA axis hyper- to hypofunction[26]. However looking at various observations this doesnt seem very likely. Hypocortisolism is only present in a minority of the patients[222], HPA axis dysfunction is not present during the early stages of ME/CFS[223] and seems to develop gradually and to be more pronounced the longer ME/CFS exists[224], whereas immune activation and inflammation are often already present at the onset of the illness[102]. In contrast, chronic inflammation and immune activation can induce adrenal exhaustion gradually through various pathways (figure 4, L1): (a) (synergistic) suppression of cortisol release and the cortisol response to adrenocorticotropic hormone (ACTH) by tumor necrosis factor alpha (TNF)[225,226], possibly mediated by NO[227], and interleukin (IL)-1[228]; (b) HPA axis desensitizing[229], resulting into a long-lasting state of LPS tolerance to a second exposure of LPS, affecting the response of plasma TNF and HPA-hormones to LPS[230]; (c) reduced adrenal response to ACTH as a consequence of elevated levels of interleukin-10[231], associated with Th2 and Treg immune responses. Adrenal responses can also be inhibited by oxidative[232,233] and nitrosative stress/NO[234,235] (Figure 4, L2). A typical endocrine abnormality in ME/CFS relates to increased sensitivity of the cellular immune system[143,144] and HPA axis[183,184] to glucocorticoids (Figure 4, M1 and M2), plausibly explaining the paradoxical combination of hypocortisolism and Th2 predominance in ME/CFS.

Mitochondrial dysfunction induced by oxidative and nitrosative stress
In addition to causing damage to lipids, proteins and DNA, inducing neo-epitopes, oxidative and nitrosative stress can have various other negative effects (Figure 5).
ROS and RNS, including ONOO-, exert multiple effects on mitochondria, including mitochondrial dysfunction, damage, and apoptosis[236,237] (Figure 5, N). NO and RNS inhibit the respiratory chain, leading to elevated O2 production, and, after reaction with NO, to increased ONOO- levels, further impeding mitochondrial respiration[238] (Figure 5, O).

Cardiovascular abnormalities due to oxidative and nitrosative stress
NO plays an important role in the cardiovascular system and increased levels of NO can have various adverse cardiovascular effects[239] (Figure 5, P). NO is a potent vasodilator[240,241] and seems essential in autoregulation of blood flow, both in large arteries and at the microcirculatory level, thereby determining the distribution of flow among the various vascular networks[239]. Therefore elevated basal NO levels in ME/CFS could explain decreased peripheral resistance and low blood pressure (hypotension)[242]. Although possibly mediated by other pathways, these phenomena are induced in healthy subjects by exercise[243]. Exercise-induced NO, added to elevated basal levels, might partially account for post-exertional hypotension and delayed recovery in ME/CFS[242]. Furthermore, elevated NO levels could also be involved in reduced myocardial contractility, disrupted autonomic modulation of myocardial function and abnormalities in heart rate variability[239,244,245]. The cardiovascular aberrations and/or mitochondrial dysfunction/damage could plausibly explain reduced oxygen uptake[158] and oxygenation[71] during exercise in ME/CFS (Figure 5, Q).

Neurological aberrations as potential consequences of inflammation, oxidative and nitrosative stress, cardiovascular abnormalities and reduced oxygen uptake
Various neurological abnormalities could be induced by the abnormalities in ME/CFS. First, systemic inflammation can induce neuroinflammation and sickness behavior[246] (Figure 5, R). Pro-inflammatory cytokines, induced by peripheral inflammation, can access the central nervous system through various pathways, and induce cytokines, amplify cytokine signals and release secondary messengers, e.g. NO, in the brain thereby influencing virtually every aspect of brain function[247]. Peripheral inflammation affects neuroendocrine function, NMDA receptor activity, neurotransmitter metabolism, and neurogenesis[248]. The behavorial effects of peripheral inflammation include depression, fatigue, psychomotor slowing, cognitive dysfunction and sleep disruption[247]. In addition to systemic inflammation-induced sickness behaviour, other mechanisms by which immunological aberrations affect the nervous system in ME/CFS have been suggested, e.g. an elevated release of cytokines by glial cells[249], auto-immune pathways, involving vasoactive neuropeptides damaging the blood-brain barrier and blood-spinal barrier[250], and infections of the nervous system by neurotropic viruses[45]. A recent study established evidence for brain glial activation in patients with chronic pain, a phenomenon repetitively observed in animal models[251]. Activation of microglia or astrocytes, related to neuro-inflammation, in widespread brain areas[174] could account for the chronic pain experienced by many patients with ME/CFS.

Second, increased levels of NO-stimulated glutamate release and hypersensitivity of NMDA receptors[252,253] could account for central sensitisation, manifesting itself in enhanced sensitivity of the central nervous system to various stimuli, e.g. sound, and hyperalgesia (Figure 5, S). Others have proposed an NO-independent O2-mediated pathway to induce hyperalgesia[254,255]. Third, cardiovascular abnormalities, resulting into reduced cerebral blood flow[70] (Figure 5, T1) and cerebral oxygenation[256] (Figure 5, T2), induced or intensified by exertion[71], would account for reduced ATP synthesis in the central nervous system, as implicated by increased ventricular lactate levels[68].

The physiological effects of exercise and stress can explain post-exertional malaise
Exercise and psychological stress could amplify pre-existing immunological abnormalities, oxidative and nitrosative stress, and intestinal hyperpermeability (Figure 6). Since these anomalies can plausibly explain various typical symptoms of ME and CFS (next paragraph), the physiological effects of exercise and psychological stress would intensify abnormalities already present in rest. This could explain a (prolonged) aggravation of symptoms after a minor exertion: post-exertional malaise.

Exercise has well-known beneficial effects[257,258]. Strenous exercise induces an increase in the pro-inflammatory cytokines TNF, IL-1, and IL-8 and the inflammatory cytokine IL-6, produced locally in the skeletal muscle in response to exercise[259,260]. This release is counterbalanced by the release of IL1 and TNF inhibitors and IL-10[259]. In chronic inflammatory diseases, exercise could have adverse effects through a combination of exercise-induced stimulation of immune signals with leukocytes previously affected by other stress, inflammatory, or immune mediators[261]. Deviant effects of exercise on inflammation and immune activation in ME/CFS (Figure 6, X1) are illustrated by the observations that the severity of symptom flare after moderate exercise is directly linked to increased levels of IL-1, IL-12, IL-6, IL-8, IL-10, and IL-13 8 hours post-exercise[188], that travelling from home to the hospital is sufficient for significantly elevated TGF- levels[262], that exercise induces a sustained increase in plasma TNF- in patients, not in controls[262], and that moderate exercise induces a larger 48 hours post-exercise area under the curve for IL-10[15]. In addition, since acute[263] and chronic[264] stress can induce inflammation[264], psychological stress could amplify pre-existing (low-grade) inflammation in ME/CFS.

Exercise can also have immunosuppressive effects. While moderate exercise seems to stimulate immunity, prolonged strenuous exercise seem to suppress immune function, e.g. NK cell activity and antibody synthesis[265,266]. Since the aerobic threshold seems (profoundly) decreased in ME/CFS, low-level anaerobic exercise can amplify immunosuppression, e.g. diminished NK cell cytotoxicity and antibody levels, observed in ME/CFS (Figure 6, X2).

In addition, eccentric exercise could induce and/or intensify immune dysfunction in ME/CFS (Figure 6, X3), e.g. inducing or amplifying a Th2-predominance[267,268]. Acute, subacute or chronic stress can suppress cellular (Th1) immunity and boost humoral (Th2) immunity, due to a differential effect of glucocorticoids and catecholamines on Th1/Th2 cells and type 1/type 2 cytokine synthesis[263]. In general, physiological and psychological stress may cause a selective suppression of Th1 functions and a shift towards a Th2 response, protecting the host from systemic 'overshooting' with pro-inflammatory cytokines[269].

Another mechanism by which exercise can amplify pre-existing abnormalities is intestinal hyperpermeability[270,271] (Figure 6, X4). Psychological and physiological stress can compromise the intestinal barrier function[272,273]. Intestinal hyperpermeability could result in translocation of enterobacteria to the blood stream, instigating inflammation in response to endotoxins.

An additional pathway by which exercise can amplify pre-existing aberrations is oxidative[274,275] and nitrosative[274,276] stress as a result of exercise (Figure 6, X5). ME/CFS seems to be associated with a prolonged accentuated oxidative stress and reduced heat shock proteins responses to incremental exercise[93]. Several observations suggest an abnormal adaptive response to exercise in ME/CFS[92], the severity of which seems to be related with premorbid physical activity and severe acute infections[150]. Through elevated glutamate release and subsequent NMDA receptor activation and induction of nuclear factor kappa-B (NF-kB), psychological distress enhances levels of NO and pro-oxidants in various brain areas[277,278].

CONCEIVABLE ETIOLOGY OF CHARACTERISTIC SYMPTOMS
The abnormalities observed in ME/CFS patients or substantial subgroups could explain the presence and variability of various characteristic symptoms (Figure 7 and Table 4). As explained in the previous paragraph the physiological effects of exercise in general and in ME/CFS in particular could account for the post-exertional malaise: exercise-induced intensification of symptoms[279].

DISCUSSION
The subjective and ambiguous criteria for CFS define a heterogeneous population of patients with chronic fatigue[32]. While chronic fatigue is obligatory for the diagnosis CFS[2], easy muscle fatigability and cognitive impairment, but above all, post-exertional malaise are mandatory for the diagnosis ME, whether defined by the original criteria[304] or the recently proposed new[1] criteria. ME and CFS are two distinct, partially overlapping clinical entities[8]. Based upon observations, it is estimated that 30-60% of people fulfilling the criteria for CFS[2] meet the more strict criteria for ME.
Fatigue is not obligatory for the diagnosis ME, and since the majority of the research in the last decades have used the CFS criteria for patient selection, it is unknown how many patients fulfilling the ME criteria dont meet the case definition for CFS. Despite the confusion created by the use of the CFS criteria, various studies have observed typical abnormalities in the ME/CFS patient group or significant ME/CFS patient subgroups.

More consistent findings relate to four types of immunological abnormalities in ME/CFS: inflammation (I1), (Th2-predominated) immune activation and counteractive immunoregulatory responses (I2), immunosuppression (I3), and immune dysfunction (I4). These immunological abnormalities and their direct and indirect sequels can account for various abnormalities observed in ME/CFS and several typical symptoms, including post-exertional malaise and weakness. ME/CFS often has an sudden, flu-like onset. Whether the original infection persists and perpetuates the illness or is only a hit-and-run infection remains subject to debate. However, using different methods and samples, various infections have been observed in substantial ME/CFS patient subgroups. Immunosuppression (I3) and immune dysfunction (I4), either due to pathogens modulating and evading the immune system (as an effect) or enabling chronic/reactivating infections (as a cause) or both, seem to play a key role in the etiology. (Chronic) inflammation (I1) and immune activation (I2), both observed repetitively in ME/CFS, can induce and sustain a vicious circle of reactive oxygen and nitrogen species and peroxynitrite. Elevated oxidative/nitrosative stress has various detrimental effects: inflammation (I1), immunosuppression (I3), immune dysfunction (I4), the generation of auto-epitopes (due to oxidative and nitrosative damage to proteins, mitochondria etc.), mitochondrial dysfunction, cardiovascular deficits, et cetera-. Gastro-intestinal dysbiosis and inflammation and intestinal hyperpermeability, found by some studies, could result into translocation of enterobacteria into the blood stream, thereby inducing a third potential immunological stimulus in ME/CFS (i.e. LPS). HPA axis dysfunction, especially hypocortisolism and HPA axis hyporesponsiveness, can explain some immunological abnormalities, but seem to arise at a later stage of the disease. On the other hand, inflammation, immune activation and oxidative and nitrosative stress can induce hypocortisolism and a blunted adrenal response to ACTH through various pathways gradually. The endocrine and immunological anomalies in ME/CFS reflect a paradox: reduced adrenal output (cortisol) combined with suppression of the (cellular) immune system, (possibly) due to enhanced glucocorticoid sensitivity of the Th1 arm of the immune system. Finally, inflammation/immune activation, cardiovascular impairment and low oxygenation/oxygen uptake could account for various neurocognitive and neuropsychological abnormalities found in ME/CFS.

Other authors have proposed alternative explanatory models for ME/CFS. The ONOO-model, with a key role for the self-perpetuating vicious circle of elevated oxidative and nitrosative stress, resulting into peroxynitrite (ONOO-), proposed by Pall et al[305] is incorporated within the 4I explanatory model. The 4I explanatory model is also in line with the NO-induced central sensitisation-model of Meeus et al[306]. The 4I model has commonalities with the neuro-immunological (NI) model for ME/CFS, put forward by Morris and Maes[307]. However, there also some relevant differences. In essence, the NI model is a linear model in which a non-persistent infection induces a vicious circle of oxidative and nitrosative stress and inflammation, neo-epitopes (induced by oxidative and nitrosative damage to proteins) and autoimmunity. The 4I hypothesis embodies key roles for reactivating and chronic infections, immune dysfunction and Th2-dominated immune activation (next to inflammation), HPA axis dysfunction, e.g. hypocortisolism and blunted adrenal responses, enhanced sensitivity of the HPA axis and the (cellular) immune system to the suppressive effects of cortisol, and circulatory deficits[3]. In contrast with the hypothesis that maladaptive stress responses, either due to a stress crash[26] or allostatic overload[308], are causing the immunological abnormalities seen in ME/CFS, the 4I hypothesis is based upon the premise that the immunological aberrations and oxidative/nitrosative stress can induce and sustain the endocrine abnormalities and defective stress responses through various pathways. The 4I hypotheses is consistent with the alternate homeostatic state-hypothesis of Craddock et al[309], although, the 4I incorporates an opposing cause-and-effect-relationship between the immunological and endocrine abnormalities in ME/CFS.

LIMITATIONS
While the abnormalities incorporated in the 4I model have been observed in ME/CFS patients or major patient subgroups repetitively, the cause-and-effect relationships between these abnormalities have often been established in general, non-ME/CFS-specific circumstances. However, the causal relations underpinning the 4I hypothesis, although speculative, collectively offer a plausible explanation for various abnormalities frequently observed in ME/CFS and characteristic symptoms.

Due to the heterogeneity of the CFS[2] patient group, the duration of the illness of the patients investigated, and the essential role of exertion-induced abnormalities, none of the abnormalities will be present in all CFS patients at all times and it is unlikely that here will ever be one abnormality present in all CFS[2] patients. In order to establish which abnormalities and causal relationships are applicable to specific patients, it is also essential to make a distinction between patients with post-exertional malaise, cognitive deficits and other characteristic symptoms of ME[1], and CFS[2] patients without these typical symptoms and to investigate well-defined biological and symptomatic ME and CFS patient subgroups in more detail.

The 4I hypothesis can be tested by investigating the abnormalities in biological and clinical subgroups defined by biomarkers and objective measures of the clinical status[3], by applying correlational analyses on the abnormalities present in patient subgroups, and by testing interventions aimed at reversing specific aberrations in patients, e.g. infections, immunological aberrations and oxidative/nitrosative stress.  

Conclusions
Immunologic abnormalities frequently observed in ME/CFS, inflammation (I1), (Th2-biased) immune activation (I2), immunosuppression (I3) and immune dysfunction (I4) seem to play a crucial role in the etiology and pathophysiology of ME/CFS. These immunological aberrations, combined with three potential immunological stimuli, can explain various other abnormalities observed in ME/CFS and underpin the 4I explanatory model for ME/CFS, which accounts for several characteristic symptoms.

Future research should confirm various abnormalities and explore the aberrations in more detail in specific clinical and biological subgroups. Correlational analysis of co-existing abnormalities in patient subgroups and the outcomes of interventions aimed at specific abnormalities could be used to accept, refine or reject the cause-and-effect relationships incorporated in the 4I explanatory model for ME/CFS.

Poster's note: My bold in text. Desriptive sections have been omitted for reasons of insufficient space for posting.

Cort and others more experienced (longer time spent in research than I): This hypothesis seems quite logical. How does it fit with what you have encountered in the literature thus far?
 

Issie

Well-Known Member
A few years ago us POTS people started looking into NO. Mayo even started testing us to see what our levels were. We convinced ourselves this was it - but for many of us that used meds and or supplements that influenced it - it didn't make that much difference. I do think it plays a part in things. But I don't think it's the whole picture. It's easy to tweak it with supplements to see if it makes a difference for you.

I do agree there are mitrocondrial issues. Dr G, my neuro at Mayo thinks so too. He has us on what he calls "the cocktail" supplements to up that function. Main thing there is CQ10. He has done testing to see if labs could detect the mitro dysfunction but it comes up null. But science is advancing - there are things science can't test completely. So he treats us any way.

I'm one of the biggest promoter of inflammatory issues. I listened to a webinar this morning on Lyme and new understandings and ways of treating it. The two docs talking were saying that they felt the severe herx reactions we get with die off is cytokine related. They are using massive doses of curcurmin for this. (I use turmeric and black cumin seed and green tea.)

As for the autoimmune system - this is my other biggie. I think this is what it all comes down to. This is the core. With me, it's not just under active in recognizing things it should be eradicating - but it also attacks me when it shouldn't. (Vitiligo and allopecia). So for sure this is dysfunctional.

I truly believe if we address our autoimmune systems and inflammation that's going to be the best we can do for ourselves. If we can figure out if there are pathways out of balance to help with those tweaks, we may be ahead of the game. I also believe what we put in our mouth and use on our bodies makes a huge difference. I'm very optimistic and jazzed. We are making advancements and great discoveries.

Thanks for the post. It was interesting to me.

Issie
 

J William M Tweedie

Well-Known Member
A few years ago us POTS people started looking into NO. Mayo even started testing us to see what our levels were. We convinced ourselves this was it - but for many of us that used meds and or supplements that influenced it - it didn't make that much difference. I do think it plays a part in things. But I don't think it's the whole picture. It's easy to tweak it with supplements to see if it makes a difference for you.

I do agree there are mitrocondrial issues. Dr G, my neuro at Mayo thinks so too. He has us on what he calls "the cocktail" supplements to up that function. Main thing there is CQ10. He has done testing to see if labs could detect the mitro dysfunction but it comes up null. But science is advancing - there are things science can't test completely. So he treats us any way.

I'm one of the biggest promoter of inflammatory issues. I listened to a webinar this morning on Lyme and new understandings and ways of treating it. The two docs talking were saying that they felt the severe herx reactions we get with die off is cytokine related. They are using massive doses of curcurmin for this. (I use turmeric and black cumin seed and green tea.)

As for the autoimmune system - this is my other biggie. I think this is what it all comes down to. This is the core. With me, it's not just under active in recognizing things it should be eradicating - but it also attacks me when it shouldn't. (Vitiligo and allopecia). So for sure this is dysfunctional.

I truly believe if we address our autoimmune systems and inflammation that's going to be the best we can do for ourselves. If we can figure out if there are pathways out of balance to help with those tweaks, we may be ahead of the game. I also believe what we put in our mouth and use on our bodies makes a huge difference. I'm very optimistic and jazzed. We are making advancements and great discoveries.

Thanks for the post. It was interesting to me.

Issie
I'm glad you found it useful, Issie.

I'm in an argument situation with our provincial Health Care agency regarding nutritional supplements. Are there any studies that could support the claim for supplements? I'd be interested to know what supplements you are taking and in what doses, if you'd like to share. Thanks.
 

Issie

Well-Known Member
Thanks for the graphics. Quite detailed and interesting in concept.

Im in the process of changing my meds and supplements. There are a whole lot of studies on CQ10 and their benefit. It is being used to halt Parkinson disease. I would recommend looking on the LifeExtension site. There are so many good articles there. Pick an illness or supplement and it will direct you to info.

Right now a few things I'm finding very beneficial is Turmeric, Black Cumin Seed, Green tea, Yucca, zinc, vit D3 and others. The others I dont take daily. Im trying to slowly get off others except for methyl B12, molybdenum, and kelp for iodine (these things are necessary for my mutations in methylation and because Im vegan.) As for meds - I'm off everything for the last two weeks now. So far, so good. That being said, keep in mind we are all different and what works for me may not work for someone else. I cant recommend something to you without knowing your full health history (nor should anyone - unless they are a professional and know the ins and outs of what is counterindicated for your unique and individual body). Also, supplements are concentrated, singled out versions of more "natural" medicines. Traditional meds usually come from singled out parts of plants or bacteria. Your body has to properly break them down, assimulate them and them hopefully give you the desired effect. Not something to take if not needed - just because - it may help. Years ago, I was of another opinion. I thought we should supplement anything considered essential as a preventative. Now - I feel we need to do it with our foods and what we put into our bodies in its whole form with everything in its perfect ratios of nutrients. Going whole food, lower fat Vegan has been a lifesaver for me.

Issie
 

Issie

Well-Known Member
I just signed up for a new site a few days ago and got the first newsletter. GreenMedInfo.com. It looks pretty interesting. has a section on pharmacological alternatives. The newsletter is free and I got into the info section just fine without a paid membership.

Issie
 

Cort

Founder of Health Rising and Phoenix Rising
Staff member
I'm looking forward to reading this. I have to say though that I think the ME vs CFS paradigm is really dated. I think we're dealing with a variety of pathways that can end up in the endpoints ME and CFS - one of which is more severe than the other but both of which contain many similar findings.It's impossible as well to legitimately state the two are different without studies showing they are different and too few studies have compared the different definitions to do that. The argument is more of a political one than anything in my opinion.
 

Issie

Well-Known Member
I'm looking forward to reading this. I have to say though that I think the ME vs CFS paradigm is really dated. I think we're dealing with a variety of pathways that can end up in the endpoints ME and CFS - one of which is more severe than the other but both of which contain many similar findings.It's impossible as well to legitimately state the two are different without studies showing they are different and too few studies have compared the different definitions to do that. The argument is more of a political one than anything in my opinion.
I agree with that statement. I think a lot of what we are calling other types of illnesses (CFS, POTS, OI, etc) may be different presentations of a very similar issue yet with different subsets in each category. One person may have high NO another low NO depends on their subset. But we are looking at symptoms as the issue when symptoms could be the compensation to the core issue and very much needed for life's preservation. Getting to the core of the problem and not focusing on the variety of compensation/symptoms is the key. Not that the symptoms are not a key part of the puzzle but figuring out why the symptoms are there in the first place. I still think autoimmune dysfunction and inflammation is the core.

Issie
 

J William M Tweedie

Well-Known Member
Getting to the core of the problem and not focusing on the variety of compensation/symptoms is the key. Not that the symptoms are not a key part of the puzzle but figuring out why the symptoms are there in the first place. I still think autoimmune dysfunction and inflammation is the core.
I agree from a logical point of view not a medical one...which I obviously don't have, not being a Dr. If one were to follow all the subset lines to the core the general research indicates they would lead to either or both autoimmune dysfunction and inflammation.

Interestingly, I saw a new Dr today (3rd visit) and he is being proactive with tests. He asked me if I had to name one symptom that characterized my condition what would it be. Good question, I thought. Fatigue was my answer as I think the pain I feel is not as consistent though it could very well be a circular argument.
 

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