phillybadboy
New Member
The difference is 2,3dpg, one had it in the serum the other did not.
Ron Davis study in mononuclear cells abnormalities possibly in glycolysis , and impedance , addition of pyruvate made the normal . 2,3dpg levels are higher in cfs . It is released from the muscle and maybe brain too into the bloodstream and taken by mononuclear cells . 2,3dpg inhibits hk, pfk, gapd . this results in lower glycolytic intermediates and pyruvate . Less pyruvate is available for oxidative phosphorylation , this is why when they added pyruvate the cells behaved normally .
the Standford study showed higher levels of atp production from nonmitochondrial sources ( probably glycolysis ). The mononuclear cells from cfs patients in this study became adapted to the inhibition by 2,3dpg , they tried to increase their activity to cope with the inhibition by 2,3dpg . when the study was done with patient mononuclear cells but not with patient serum ( the 2,3dpg is in patient serum ) the 2,3dpg inhibition of the glycolytic enzymes has been lifted so that now the glycolytic enzymes became overly activated , hence the higher levels of atp through higher levels of glycolysis.
Ron Davis study in mononuclear cells abnormalities possibly in glycolysis , and impedance , addition of pyruvate made the normal . 2,3dpg levels are higher in cfs . It is released from the muscle and maybe brain too into the bloodstream and taken by mononuclear cells . 2,3dpg inhibits hk, pfk, gapd . this results in lower glycolytic intermediates and pyruvate . Less pyruvate is available for oxidative phosphorylation , this is why when they added pyruvate the cells behaved normally .
the Standford study showed higher levels of atp production from nonmitochondrial sources ( probably glycolysis ). The mononuclear cells from cfs patients in this study became adapted to the inhibition by 2,3dpg , they tried to increase their activity to cope with the inhibition by 2,3dpg . when the study was done with patient mononuclear cells but not with patient serum ( the 2,3dpg is in patient serum ) the 2,3dpg inhibition of the glycolytic enzymes has been lifted so that now the glycolytic enzymes became overly activated , hence the higher levels of atp through higher levels of glycolysis.
![ME/CFS lecture | Dr Nancy Klimas | 2011 [Chronic Fatigue Syndrome / SEID / New Zealand] - YouTube](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)
