Transient receptor potential melastatin 3 ion channels on Natural Killer cells and B lymphocytes

Discussion in 'Chronic Fatigue Syndrome (ME/CFS) Research' started by h3ro, May 31, 2016.

  1. h3ro

    h3ro Active Member

    Novel identification and characterisation of Transient receptor potential melastatin 3 ion channels on Natural Killer cells and B lymphocytes: effects on cell signalling in Chronic fatigue syndrome/Myalgic encephalomyelitis patients

    • T. Nguyen,
    • D. Staines,
    • B. Nilius,
    • P. Smith and
    • S. Marshall-Gradisnik

    Biological Research201649:27
    DOI: 10.1186/s40659-016-0087-2

    © The Author(s) 2016

    Received: 26 January 2016

    Accepted: 9 May 2016

    Published: 31 May 2016


    Transient receptor potential melastatin 3 (TRPM3) cation channels are ubiquitously expressed by multiple cells and have an important regulatory role in calcium-dependent cell signalling to help maintain cellular homeostasis. TRPM3 protein expression has yet to be determined on Natural Killer (NK) cells and B lymphocytes. Multiple single nucleotide polymorphisms have been reported in TRPM3 genes from isolated peripheral blood mononuclear cells, NK and B cells in Chronic fatigue syndrome/Myalgic encephalomyelitis (CFS/ME) patients and have been proposed to correlate with illness presentation. The object of the study was to assess TRPM3 surface expression on NK and B lymphocytes from healthy controls, followed by a comparative investigation examining TRPM3 surface expression, and cytoplasmic and mitochondrial calcium influx in CD19+ B cells, CD56bright and CD56dim cell populations from CFS/ME patients.

    TRPM3 cell surface expression was identified for NK and B lymphocytes in healthy controls (CD56bright TRPM3 35.72 % ± 7.37; CD56dim 5.74 % ± 2.00; B lymphocytes 2.05 % ± 0.19, respectively). There was a significant reduction of TRPM3 surface expression on CD19+ B cells (1.56 ± 0.191) and CD56bright NK cells (17.37 % ± 5.34) in CFS/ME compared with healthy controls. Anti-CD21 and anti-IgM conjugated biotin was cross-linked with streptavidin,and subsequently treatment with thapsigargin. This showed a significant reduction in cytoplasmic calcium ion concentration in CD19+ B lymphocytes. CD56bright NK cells also had a significant decrease in cytoplasmic calcium in the presence of 2-APB and thapsigargin in CFS/ME patients.

    The results from this preliminary investigation identify, for the first time, TRPM3 surface expression on both NK and B lymphocytes in healthy controls. We also report for the first time, significant reduction in TRPM3 cell surface expression in NK and B lymphocytes, as well as decreased intracellular calcium within specific conditions in CFS/ME patients. This warrants further examination of these pathways to elucidate whether TRPM3 and impaired calcium mobilisation has a role in CFS/ME.

    Chronic fatigue syndrome Transient receptor potential Calcium signalling Myalgic encephalomyelitis
    bobby, Cort, Tony L and 1 other person like this.
  2. Cort

    Cort Founder of Health Rising and Phoenix Rising Staff Member

    I love hearing about ion channels! I wrote a blog on a neurological channelopathy in ME/CFS when I was with PR. A fascinating topic. Ion channels are big in chronic pain by the way. Glad the Aussies are digging into this.