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(This is a lengthy blog. Now might be the time, if you haven’t used them yet, to use the print or PDF buttons at the bottom of the blog)

Recently a Senate Subcommittee zeroed out the entire budget of the CDC’s CFS program for next year in an Appropriations bill. It’s not clear why they did so, but initial reports suggest that chronic fatigue syndrome’s lack of representation on the Hill may have lead the subcommittee to believe it would be an easy mark. (Note that the Infectious Disease section of which chronic fatigue syndrome is a part received about a ten percent increase in funding.)

Losing the CDC program would cut in half the research dollars the federal government devotes to ME/CFS.  Upon learning of the possible funding cuts Jennie Spotila’s question – “Why not just let the CDC program go?” – sparked an at times furious debate. That debate sparked me to do something I’d promised to do for a long time – a complete overview of the CDC’s research program.

A Controversial Program

If anything is a hot-button topic in ME/CFS the Centers For Disease Control’s CFS program is. From the sometimes troubling portrayal given it  in Osler’s Web, to the misappropriation of ME/CFS funds in the 1990’s, to a “Toolkit” with few tools in it, to the Empirical definition with its huge and never fully explained jump in prevalence, to Dr. Reeves intemperate (although ultimately correct) remark that XMRV would not work out, the CDC has had a talent for getting itself in hot water with the ME/CFS community.

CDC CFS Funding ended

With a stroke of the pen the CDC program on ME/CFS disppears (!)

Dislike of the program can be intense. Recently participants in a heated discussion stated things like the CDC has never studied pathogens, and that the CDC has never done a single good thing for the ME/CFS community. Another person in another email exchange stated that the CDC had spent millions on CBT studies.

None of the above are true.  The CDC has done more pathogen studies than any other group, it has never done a CBT study, and it has produced numerous studies that have contributed to our understanding of ME/CFS.

This is not to say that the CDC team has not made mistakes – some of them major — or done things which have contributed to unhelpful impressions of this disease – but some misunderstandings about the program clearly exist.

In an effort to assess the CDC’s impact on the research field – good and bad – this blog reviews virtually every research study that Reeves and other major figures in the CDC team have published since around 1995.

The CDC’s Research Program: A Review

Bill Reeves

It all begins with the bald one. Next to Simon Wessely, Bill Reeves has probably been the most polarizing figure in ME/CFS history.

Bill Reeves

Reeves cut a striking figure – but temperamentally may not have been best choice to lead the CFS program

Reeves, an epidemiologist, brought a strong viral background to his work at the CDC’s CFS team. From 1977 to 1989, he worked in virology and epidemiology at the Gorgas Memorial Laboratory in Panama. While there, Reeves produced the first large population study to find a link between sexually transmitted viruses and cervical cancer. From 1982 through 2005 Reeves published almost 60 studies on uterine cervical cancer.  His work was later described as being fundamental in establishing the link between human papillomavirus and uterine cervical cancer.

Reeves with his bald pate, fierce glare and military manner may not have been the best choice temperamentally, to run a program for the ME/CFS community. A fairly gruff individual, Reeves was not inclined to communicate much. Despite being on the federal committee for ME/CFS (CFSAC) for years the committee gave him a no-confidence vote during his ten year review.

The first part of the blog is focused on papers on which Reeves was listed as co-author. Later sections of the blog feature other studies the CDC funded while he lead it. Finally the blog looks at Dr. Unger’s work.

Epidemiological Studies

Reeves was trained as an epidemiologist and did many large epidemiological studies. His first epidemiological study used physician referrals in four cities to collect epidemiological data on CFS patients. It found that the mean age of onset was 30 years old, 85% were women, 97% were white and average duration was almost 7 years.

Reeves second large epidemiological study screened 8,000 households in San Francisco for chronic, idiopathic (unexplained) fatigue. The study rarely found ME/CFS-like conditions in people under 18, but the study suggested it was surprisingly common in adults (2%). Reeves noted that symptom patterns in people who meet the criteria for CFS were similar to those who experienced fatigue but did not. This question – where to split off CFS cases from CFS-like cases – would come up again and again in Reeves studies.

epidemiology

Reeves epidemiological studies helped establish ME/CFS as a common and serious disorder

Another 2003 population-based study underscored the need for symptom relief in the disease, with CFS patients being more likely to use pain relievers, hormones, antidepressants, benzodiazepines, gastro-intestinal, and central nervous system medications.

2004 was a hallmark year in Reeves epidemiological work with several important papers coming out.  A nationwide 2004 study suggesting that 2.2 million Americans had ME/CFS (Fukuda definition) indicated ME/CFS was a  common disorder affecting many.  The authors noted, though, that after a more thorough examination some would not meet the criteria.

Another 2004 paper highlighting the high economic costs of the disease estimated that lost productivity due to ME/CFS accounted for $9.4 billion in economic losses a year.  With only 16% of the patients the CDC identified as having ME/CFS being diagnosed, a 2004 paper indicated the urgent need for better educated doctors. This number would be used again and again to call for better physician education.

  • VERDICT – Significant positive impact. Reeves epidemiological studies are still used to demonstrate that ME/CFS is a major disorder with significant unmet needs.

Pathophysiology

Pathogens

Early studies (pre-Reeves) in 1992 and 1993 failed to find evidence that HTLV or other retroviruses were present in ME/CFS. Reeves first paper on CFS reflected an initial interest in the role viruses play in the disorder. Despite testing for a “large number of infectious agents” or antibodies to a “wide range of agents including herpesviruses”, however  Reeves 1995 seroepidemiological paper found no evidence of infection. That would begin a dispiriting pattern at the CDC.

pathogens

In every one of 10 studies, the CDC failed to find evidence of pathogens in ME/CFS

Over the next four years Reeves would look for herpesviruses, bacterial agents, mycoplasma and endogenous retroviruses in ME/CFS. Neither PCR nor DNA tests found evidence of HHV6 or HHV7 in 26 CFS patients vs 52 controls.  A search for an endogenous retrovirus turned up empty as well.  A cell-free DNA study found no evidence of bacterial infection in 35 CFS vs 55 controls.  The results of a mycoplasma study lead by Suzanne Vernon were similar.

In 2005 a small study failed to find evidence of GBC-virus in ME/CFS patients. That was it for Reeves and pathogens until th.  Failure after failure would cause Reeves to swear off the pathogen search until XMRV came along – and thee CDC’s 2010 study found no evidence of it in ME/CFS patients and healthy controls.

Reeves appears to have become convinced that pathogens did play a role -but as a triggering factor – not a perpetuating one.  In the mid-2000’s Reeves helped fund at least four of the seminal Dubbo studies which examined the role pathogens play in triggering ME/CFS. One of those studies indicated, probably at no surprise to Reeves by then, suggested that increased viral load was not responsible for the prolonged illness the ME/CFS patients were experiencing.

Immune System

The CDC has produced quite a few immune system studies but Reeves imprint only appeared on a few. As with the pathogen studies getting a positive outcome was not easy.

An early paper on the immune response found no or only marginal differences in a wide variety of immune factors in Reeves 1997 study. When Reeves subgrouped the patients according to onset some subtle differences emerged and Reeves proposed that disease onset should be assessed in ME/CFS studies.

In 2005 a Vernon/Reeves search for autoantibodies and antibodies to neurons failed to find significant differences between ME/CFS patients and healthy controls. Reeves and Suzanne Vernon co-authored a Dubbo study that failed  to find evidence of cytokine upregulation in post-viral ME//CFS patients.  (Another, however, found some evidence of altered gene expression. )

A rather uninspiring 2009 peripheral inflammation study found that inflammation was increased in CFS and CFS-like patients relative to healthy controls and was associated with reduced physical functioning in CFS, CFS-like and healthy controls.

HPA Axis

Reeves first foray into genetics in 2007 revealed multiple polymorphisms in the NR3C1 gene regulating the HPA axis. The study suggested CFS patients could be genetically prone to HPA axis instability.  A 2008 study finding reduced morning  salivary cortisol levels in women but not men helped to solidify the understanding that salivary cortisol is low in ME/CFS but only in the morning.

Stress

Later in his time at the CDC Reeves focused more on impacts to the stress response system.  Some CDC researchers  conceived the idea broad breakdowns in  the bodies homeostatic mechanisms  (allostatic stress) were responsible for the altered cortisol and aldosterone level found. A 2009 allostatic load paper appeared to represent a turn towards fashioning CFS as a ‘stress-disorder.

A 2010 paper –  which Suzanne Vernon told me was hotly debated in the CDC CFS group – asserted that metabolic syndrome was common in ME/CFS.  In 2011 Reeves found that “cumulative life stresses” were increased in ME/CFS and proposed that “stress may be an important factor in the pathophysiology of CFS”.  Several studies in the Pharmacogenomics studies would find evidence of increased allostatic stress.

  • VERDICT – Some positive impact. The allostatic stress studies went nowhere and gave the impression that while Reeves was fiddling around the edges of ME/CFS – looking for some grand, if somewhat vague explanation of it – other researchers were digging deeper.  Reeves did some work on the immune system – and the neuronal antibodies study was years ahead of it’s time –  but he doesn’t appear to have been particularly interested in it and it showed. Reeves tried hard on pathogens – you can’t begrudge him the effort – but was unsuccessful.  If unusual pathogen activity does turn out to be a key factor in ME/CFS, he will, of course, have missed the boat on that one – but he will join many others in doing that.

Empirical Definition

Reeves early recognized that the Fukuda definition was problematic and initially prepared for a new definition in an organized and collaborative manner. Over several years Reeves brought together an international group ME/CFS experts to assess the issues with the Fukuda Definition and provide solutions.

ME/CFS, it appeared, was about to enter a new era of study using an improved and widely accepted new definition. Reeves then sabotaged his efforts by developing a new definition in house at the CDC. The definition used scores from patient assessment tests such as the SF-36, MFI and CDC symptom inventory to produce an “empirical” assessment of CFS-ness.

Reeves - empirical definition

Reeves failure to produce a definition the research community would embrace left the Fukuda definition in play for another ten years.

With even Peter White publicly stating some doubts, however, the “empirical definition (ED)” was controversial from the start. A startling 6-10 time increase in prevalence sent shock waves through the ME/CFS community. That study suggested that 2.4% of the American population had “CFS” … and validated some researchers doubts about it.

Leonard Jason would subsequently several studies highlighting problems with the definition including the possibility that the definition could misdiagnose about a third of people with depression. Jason’s data mining study, however, found similarly unimpressive findings for both the ED and the Canadian Consensus Criteria. When asked to identify a new patient the ED did so correctly 68% of the time and the Canadian Consensus Criteria did so 73% of the time. The ED correctly identified 79% of already established cases correctly, while the CCC identified 87%.

Whether it wasn’t trusted or too complicated or whatever the ED  flopped.  Outside of the CDC it doesn’t appear to have been used by another research group.  More importantly, a major opportunity to reform and strengthen the ME/CFS research field had failed.  The  Fukuda definition would continue to be the definition of choice for ME/CFS researchers for at least the next ten years.  Not until the IOM panel  convened would a group with significant clout attempt to produce a new definition.

By around 2007, Reeves studies now had two question marks against them.  The fact that a significant portion of the CFS patients selected by population sampling no longer met the criteria for CFS several years later suggested he was studying a “CFS-lite” group. The advent of the ED raised further questions about what kinds of patients were showing up in CDC studies.

  • VERDICT – Negative Impact. A major missed opportunity for CDC program and for the ME/CFS field itself.

Symptom Assessment

Reeves introduced two improvements to symptom assessment in ME/CFS.

A 2005 paper demonstrated Reeves attempt to broaden the symptom assessment beyond the symptoms than found in the 8-symptom Fukuda criteria. The CDC Symptom Inventory assessed the presence, frequency and intensity of 19 symptoms including diarrhea, fever, chills, sleeping problems, nausea, stomach or abdominal pain, sinus or nasal problems, shortness of breath, sensitivity to light, and depression.

Reeves continued to broaden the instruments used to assess ME/CFS with a 2009 study validating the use of the Multiple Fatigue Inventory (MFI) in the disease.

  • VERDICT – Positive Impact in an important area that needed and still apparently needs help.

Symptoms and Conditions

Reeve’s studies assessing the prevalence and cause of several important symptoms/conditions met with mixed results.

In 2004 a factor analysis study found three symptom clusters – musculoskeletal, infection and cognition-mood-sleep  in the disease. The clusters overlapped, however, between people who meet the criteria for ME/CFS and those who had ME/CFS-like symptoms but did not meet the criteria. That suggested that other than symptom severity there was little dividing line between the two – at least with regard to symptoms.

Sleep

A large Unger-lead study suggested that most people with ME/CFS had a least one sleep abnormality but noted that sleep studies were needed to confirm the findings. A 2006 sleep study indeed, found that 18% of the people thought to have CFS had a primary sleep disorder instead and that and rates of sleep apnea were also increased.

Similar sleep architectures  between the CFS patients and the healthy controls, however, provided no clues to the sleep problems CFS patients reported.  A 2007 sleep study employing population based subjects and the empirical definition also found no differences in sleep pathology or architecture  in CFS patients vs control.

sleep problems ME/CFS

Reeves tried hard with sleep. He finally hit pay dirt with his HRV study.

A 2007 population-based sleep study, however, hit pay dirt. Boneva, in a finding that would be validated by another lab, found increased heart rates and reduced heart rate variability during sleep in a population-based set of patients. Those findings along with  higher norepinephrine levels and lower plasma aldosterone suggested “a state of sympathetic predominance was present” in ME/CFS.

Reeves continued to examine sleep. In 2009 he found widespread albeit modest differences in brain-wave patterns (significantly lower theta, sigma, and beta spectral power during stage 2, Slow Wave Sleep, and REM) during sleep. Among others these findings also suggested problems with the sympathetic nervous system might be important.

Other Conditions

Reeves population-based 2006 cognition study indicated that people with ME/CFS were mentally fatigued and that the mental fatigue was associated with poorer performance on cognitive tests.

A 2005 orthostatic intolerance  study, however, fared poorly. It found that none of the “CFS” patients in the study exhibited orthostatic intolerance in the standing test.  Thirty percent of the ME/CFS patients had postural orthostatic tachycardia syndrome on the tilt test but 50% of health controls demonstrated “orthostatic instability” as well (much of it due to neurally mediated hypotension.)  Reeves concluded that some form of orthostatic intolerance was common in the population and was not a major factor in CFS.

  • VERDICT – Some positive impact. Reeves seems to have pinned down a significant cause of sleep problems but his work on validating symptoms/conditions in ME/CFS in general was uninspiring and not particularly to say the least.

Behavioral Studies – the Heim-Nater Collaborations

Reeves first behavioral study did not occur until later in his stay at the CDC.  Reeves would never fully abandon his efforts to understand ME/CFS on a pathophysiological level; over time, though, behavioral studies would fill out more of the CDC’s study portfolio. Reeves association with Christine Heim  and Urs Nater produced a number of controversial studies that would solidify his reputation in the ME/CFS community as the prime bogeyman.

CDC psychological studies ME/CFS

Reeves psychological studies garnered enormous attention

A 2006 study found more “escape avoiding behavior”; i.e. “maladaptive coping” in CFS and CFS-like patients.   Reeves 2006 childhood trauma study suggested that childhood trauma was a “major risk factor” for ME/CFS.  A 2009 study with similar findings suggested that low cortisol levels were associated with a tendency for childhood trauma. Because childhood trauma is associated an increased prevalence of autoimmune, inflammatory and mood disorders, the studies did in an odd way legitimize CFS as a disease,  but they were done at the wrong time at the wrong place.  For an ME/CFS community looking for pathophysiolgical answers they were a considerable step backwards.

A 2009 population study suggested that almost 60% of people with CFS had at least one current psychiatric diagnosis and almost 90% had been diagnosed with one in their lifetime. (When ME/CFS is so often misdiagnosed as depression one wonders what relevance a study like this has.)  A 2010 study found that most people with ME/CFS (71%) did not have evidence of a personality disorder but that ME/CFS was associated with “an increased prevalence of maladaptive personality features and personality disorders”.

  • VERDICT – Negative Impact: – the psychological studies did nothing to further our understanding of ME/CFS pathophysiology.  While the personality study did find evidence of a personality disorder in 29% of patients – that still left over 70% of patients without one.  Nor did the childhood trauma studies, except perhaps by obliquely relating ME/CFS to other inflammatory disorders with similar findings, provide any clues as to pathophysiology.  The second childhood trauma study did highlight cortisol, but it’s hard to imagine that the mildly low cortisol findings found in ME/CFS are anywhere near ground zero for this disease. Except for the childhood trauma studies much of this work had already been done anyway. It was another of the CDC playing mop up.

A Complex Figure

Reeves then was a complex figure.  Coming out of the human papillomavirus field, Reeves initially believed that pathogens caused ME/CFS.  When that didn’t work he suspected that homeostatic mechanisms  in the body had broken down. He appeared to be leaning at the end to the idea that behavioral responses to the illness were contributing to those homeostatic breakdowns.

We shall see, though, that Reeves’ funding of the open ended and exploratory Pharmacogenomics studies indicated that he was not a closed book on ME/CFS.  His funding of Dubbo studies turned out to be prescient as well. Despite his turn towards the psychological at the end when Reeves was replaced he was still funding large pathophysiological studies. It’s hard to know exactly what he thought about ME/CFS.

It’s hard not to escape the conclusion that Reeves, himself, was not a particularly creative thinker or researcher. Many of his research efforts are not particularly inspiring and he devoted considerable efforts to validating (some might say invalidating) findings from other groups – as if the CDC program presented some sort of  seal of approval. His tenacity with the sleep research, however, was admirable.

Reeves did do much to put ME/CFS on the map.  His whistleblower testimony brought millions of dollars of funds back into the ME/CFS program and initiated the first media campaign the CDC had ever done. His epidemiological studies indicated ME/CFS was a common and costly disorder that was poorly diagnosed. He never stopped arguing the ME/CFS was a serious disorder.

He also used expensive population studies to find patients who fit a questionable definition. It could be argued that Reeves sampling strategy was backwards – he would have been more successful if he had developed a more stringent definition based on the patients in clinics first – and then applied it to the population at large.  Instead his application of a vague definition to the population at large appeared to cause him to end up with a “CFS-lite” group in his studies.

Reeve’s development of the Empirical Definition could almost be described as tragic. Seemingly just steps away from developing a definition  that would be embraced by the ME/CFS community -and take over for the much maligned Fukuda definition – Reeves turned inward and developed a definition that was never embraced. An major opportunity to move the entire ME/CFS field forward was lost.

That’s not the end of the Reeves story, though. Reeves also funded significant numbers of pathophysiological studies and allowed another member of his team focused on pathophysiology, Suzanne Vernon, to do much work

Back to Biology – Vernon and Unger Studies

An examination of Reeves legacy is not complete until we examine the work of Suzanne Vernon, a researcher Reeves brought onto the team from his human papillomavirus work. With her intense interest in complex statistical analyses, gene expression and the immune system, Vernon brought a different slant to the CDC’s work, and Reeves does not appear as a co-author on many of her studies.

As was noted earlier Vernon was the lead author (and Reeves was a co-author) of studies looking for mycoplasma, bacterial DNA, neuronal antibodies, glucocorticoid polymorphisms and molecular profiling.

Suzanne Vernon

Part of Reeves legacy involves Suzanne Vernon a creative research focused on the pathophysiology of ME/CFS.

A pre-Reeves study (senior author Jones) had found evidence of complement activation in the blood during exercise in 2003. Vernon’s first “solo” (non-Reeves) effort in 2005  examined the effects of exercise as well. It found greatly increased activity of ion transport and ion channel genes after exercise – two subjects that are of great interest today in pain and fatiguing diseases. A 2009 exercise study found enzymatic abnormalities that could account for the complement upregulation in ME/CFS during exercise.

In 2007 Vernon built a model which showed how the HPA axis might flip into an alternate suboptimum stat similar to that found in ME/CFS.  In 2008 she and Broderick introduced neuroimmune network modeling to ME/CFS.  In 2009 she and Broderick produced a model demonstrating how a period of short-term cortisol depletion could help reset the HPA axis.  The Nova Southeastern team under Broderick would greatly expand on these types of models.

A 2008 CDC study suggested that polymorphisms concentrated on one serotonin receptor gene could play a role in ME/CFS.  In 2008 Vernon also co-authored a study combining gene expression and gene polymorphism data in ME/CFS.

A 2009 study using the empirical definition was the first to identify B-cell abnormalities in ME/CFS. It suggested a B-cell dysfunction might be producing persistent inflammation and altering NK and T cell activity. Another 2009 study suggested that polymorphisms in complement genes could be contributing to the complement activation seen during exercise in an earlier study.

Vernon’s first attempt at integrating large amounts of disparate data (gene expression, clinical and epidemiological data) suggested that fundamental metabolic perturbations might be present  in ME/CFS.

Besides her ME/CFS work Vernon did substantial work while at the CDC improving methodological procedures associated with gene expression and mass spectrometry.   They included work on micoarrays, improving mass spectrometry data, optimizing assays, improving mass spectrometry (again), assessing gene expression kits, assessing gene expression effectiveness (7 studies).

The Pharmacogenomics studies would be her chief legacy, however.

The Pharmacogenomics Studies

The almost $13 million swiped from the CDC’s CFS program was returned to it over several years in the early to mid-2,000’s. A significant amount of that money appears to have gone to a novel Vernon lead effort that came to be called the Pharmacogenomic’s studies.

These studies represented a creative effort to redefine and understand ME/CFS.  They were amongst the first studies in any disease to use advanced statistical techniques to simultaneously assess huge data sets of epidemiologic, clinical and laboratory data. They included ME/CFS researchers outside the CDC such as Nancy Klimas, Vollmer-Conna, White and Broderick and brought in other researchers from statistical fields.

In 2006 the studies filled an entire issue of the Pharmacogenomics Journal and prompted the head of the CDC to publicly assert in a National Press Club meeting that CFS was a real disorder.

gene expression

Reeves ended much of his support for pharmacogenomics type work but it lives on in Nancy Klima’s Institute

Among others the project produced classes of ME/CFS patients using gene expression data, identified gene polymorphisms associated with those classes and associated the expression of certain genes with specifc fatigue measures.  One study combining symptom and fatigue measures with gene expression data suggested that oxidative stress, immune system dysfunction and potassium imbalance were associated with impaired sympatho-vagal balance (low HRV) in ME/CFS. Another study using a complex set of variables identified 24 genes governing immune response, apoptosis, ion channel activity, cell growth and neuronal activity in ME/CFS. Several other studies focused on allostatic load denoted by increased waist:hip ratio, low cortisol and aldosterone levels.

One intriguing study found that polymorphisms in just five genes (neuronal tryptophan hydroxylase (TPH2), catechol-O-methyltransferase (COMT) and nuclear receptor subfamily 3, group C, member 1 glucocorticoid receptor (NR3C1)) allowed investigators to accurate predict who had or did not have ME/CFS.

It was a promising start, but Reeves axed most of the further pharmacogenomics studies. The effort would live on in two places, though: Broderick would use similar methods to form the core of Dr. Klimas’s research program and Unger would retain some investigators from this period.

  • VERDICT – Significant positive impact. Vernon picked on the exercise findings in ME/CFS and expanded them and she was successful in doing that.  She was the first person to find B-cell abnormalities in ME/CFS. and the first to use sophisticated statistical techniques to analyze large amounts of data. The Pharmacogenomics project laid the basis for one of the more successful endeavors in ME/CFS research –  Klimas/Broderick work at NSU in ME/CFS and GWS. This line of research is one of the few that the federal government appears to have consistently funded.

Dr. Elizabeth Unger Takes Over

After Reeves unexpected sacking (he had recently survived a ten-year overview of the program) Dr. Unger took over as CFS program director in 2011.  Her concentration in ME/CFS prior to taking over was almost wholly biological. She, like Vernon, also tended to work on technical methodological studies focused on gene expression technology. She was rarely listed, however, as the lead or senior author on her ME/CFS studies.

Elizabeth Unger CDC CFS Chief

Unger took over a program with lots of baggage

She had, however, co-authored hundreds of papers on the human papillomavirus and was not uncommonly the senior author listed.  (Note that the major players of the CDC CFS research team – Reeves, Vernon and Unger –  came out of the papillomavirus field.)

Unger has now been directing the CDC’s CFS program for four years. The CDC’s pace of publication has slowed since Unger took over, possibly because she’s been devoting so much time to the large and data–rich multi-site studies. Since taking over as head of the program she has co-authored ten papers, three of which probably began during Reeves term.

Unger’s Studies

An  early menopause and other gynecologic risk factors study using the empirical definition found, as did an earlier CDC study using the Fukuda definition, very high levels of gynecological disorders either preceding or following ME/CFS.

A basal ganglia study (Decreased basal ganglia activation in subjects with chronic fatigue syndrome“) piggybacked on Andrew Miller’s intriguing findings that hepatitis patients given interferon – a powerful immune agent – came down with similar symptoms as people with ME/CFS. The study found evidence of basal ganglia dysfunction and reduced dopamine activity. Miller suggested those factors could result in central nervous system hypersensitivity to inflammation. Miller’s finding suggested hat inflammation plays a strong role in ME/CFS.

Unger was the senior author of the first ME/CFS paper to comprehensively assess single nucleotide polymorphisms (genetic  variants) in immune and inflammatory genes. Remarkably, the study singled out polymorphisms in the complement system – the same part of the immune system that two earlier CDC exercise studies had highlighted. This is the first time I am aware of that one immune factor has been highlighted in gene expression, the blood and genetic ME/CFS studies.

A complex study attempting to get at the cause of the natural killer cell dysfunction in ME/CFS examined the effects of acute stress on the epigenetic expression of a gene associated with perforin.  Perforin plays a key role in natural killer cells ability to kill infected cells. The study had somewhat mixed results but did suggest that increased methylation could be altering perforin gene expression and thus contribute to the NK cell problems found in ME/CFS.

In 2011 Unger co-authored a study integrating single nucleotide polymorphism and gene expression results in ME/CFS. The study found two genes, a glutamate regulating gene and a neuronal gene, which displayed both altered gene expression and unusual gene variants.  Unger also produced a 2011 paper suggesting that a serotonin receptor polymorphism plays a role in altered methylation processes and ultimately serotonin receptor activity in ME/CFS.

Under Dr. Unger, the CDC is the first group, as well, to finally provide an objective assessment of Dr. Chia’s findings. They are current analyzing samples from Dr. Chia’s enteroviruses.

Unger also co-authored two review papers:  one on current and emerging biomarkers and one on the minimum data elements needed for research studies in ME/CFS and two methodology papers (Multiscale analysis of heart rate variability in non-stationary environments, Identification of Phosphoglycerate Kinase 1 (PGK1) as a reference gene for quantitative gene expression as well as a study producing a pilot registry.

VERDICT – Positive Impact  Unger at this point may have the highest success rate of any of the major CDC researchers. The gynecological studies produced startling evidence that high rates of gynecological disorders are present in ME/CFS – something Broderick’s models suggest might happen. The basal ganglia study, in fascinating fashion, pointed a finger straight at the immune system and the brain.  While Unger’s attempt at explaining the NK cell problems in ME/CFS did not appear to me (a laymen in this complex topic) to be particularly successful it was a worthy effort. Her immune/inflammation polymorphism study,  in what may turn out to be a major finding, highlighted the complemennt system again.  Time will tell whether glutamate plays a role in this disease or not.

The most significant dig on Unger’s directorship is probably that it has not been particularly prolific. Nor has it been particularly speedy. She presented preliminary results of the first multi-site study about two years ago yet no papers have been published. Unger also continues using the ED but it’s time is surely coming as Jason’s studies bring us closer and closer to the best criteria for ME/CFS.

 Conclusion

Unger has put a strong focus on biology

Unger has put a strong focus on biology

Unger charted a different path from Reeves. She stopped the stress and psychological papers and began a focus on immune factors and using the genetics and gene expression techniques the CDC team had helped to create.  The gene focus continued with the CDC’c presentation at the IACFS/ME conference indicating telomerase lengths were reduced in ME/CFS and aging may be increased.

The results of Unger’s main work since she took over, though – the multi-site ME/CFS experts study – with its exercise, cognition, lactate, gene expression and salivary cortisol components – have yet to be published.  The study will likely contribute to our understanding of diagnostics, comorbidity, family history, disease course and treatment effectiveness.  Phase II of the study is examining large numbers of severely ill patients and pediatric patients.

Achievements of the CDC’s CFS Research Team

Contrary to popular opinion the CDC’s research team has contributed to our understanding of ME/CFS. Below are some of the ways it has done that:

Epidemiology

  • Determined that ME/CFS causes severe direct yearly economic losses in the U.S.
  • Determined that the vast majority of people with CFS are undiagnosed
  • Produced studies indicating that ME/CFS is a relatively common disorder in the U.S.
  • Produced the first pediatric prevalence study in the U.S.
  • Identified that the highest rates and chances for remission occur in the first two years of the disease

Symptom Assessment

  • The CDC’s Symptom Inventory increased the number of symptoms assessed in CFS evaluations and added frequency and severity measures
  •  the CDC’s  Multi-Fatigue Inventory presented an improved symptom assessment instrument

Pathogens and Immune System

  • Found no evidence of pathogen persistence in ten studies over two plus decades
  • Provided funding for the seminal Dubbo studies that identified infections are common triggers of ME/CFS  and that symptom severity and cytokine upregulation early in the infection are key factors. (Reeves, Vernon were co–authors on several papers)
  • Produced four studies suggesting that complement activation may play a factor in the ME/CFS – particularly during exercise in ME/CFS
  • Produced the first gene expression study to find B-cell abnormalities in the blood
  • Several Pharmacogenomics studies pointed to possible immune system abnormalities

Symptoms and Conditions

  • Identified, in two studies, abnormally high rates of gynecological disorders in women with ME/CFS
  • Identified that as many as 25% of people meeting the criteria for ME/CFS may instead have a primary, untreated sleep disorder that would show up in a sleep study.
  • Identified sleep apnea as a common and undiagnosed cause of fatigue in ME/CFS patients
  • Produced the first study to indicate that reduced heart rate variability (i.e. sympathetic nervous system dysfunction) played a key role in the poor sleep seen in ME/CFS. That finding was later validated.
  • Found that ME/CFS patients were mentally fatigued and that mental fatigue was linked to reduced cognition

HPA Axis

  • Identified and validated what turned out to be the core HPA axis finding in ME/CFS – low morning salivary cortisol levels

Genetics, Gene expression, Data Mining and Modeling

  • Produced the first neuroimmune network modeling study in ME/CFS. (The study suggested  inflammation and thyroid dysfunction are present in ME/CFS.) Network modeling has played a major role in Dr. Klimas’s later work.
  • Produced the first model of HPA axis instability suggesting how HPA axis problems occurred in ME/CFS and how the axis might be reset.
  • Produced the first study combining genetic and gene expression data in ME/CFS
  •  In 2006 CDC studies filled an entire issue of the Pharmacogenomics Journal. Upon publication of the journal the head of the CDC announced in a National Press Club event that CFS was a real disorder.
  • Pharmacogenomic studies identified immune, ion channel, oxidative stress factors that remain pertinent in today’s research
  • Produced a dual gene polymorphism/gene expression study suggesting abnormal glutamergic neurotransmission may be occurring in ME/CFS.
  • Demonstrated that abnormal methylation of perforin genes may be occurring in ME/CFS
  • Found that reduced telomerase lengths in ME/CFS suggest more rapid aging

Brain

  • Produced a study demonstrating that people with ME/CFS have similar basal ganglia dysfunction and symptoms as hepatitis patients given a strong pro-inflammatory drug. This finding strongly suggested that inflammation plays a major role in ME/CFS.

Others

  • Reeves introduced Suzanne Vernon to the ME/CFS field. Vernon went on to successfully revamp the Solve ME/CFS Initiative’s research effort, build the Biobank and introduce many new researchers to the field. Reeves also introduced Elizabeth Unger who has refocused the CDC’s CFS program on pathophysiology to the field. The Pharmacogenomics efforts also appear to have introduced Gordon Broderick to the field.

An Assessment

The CDC did produce numerous accomplishments over the course of its 15 or so years of work. Did it produce enough? I would suggest that the program has underperformed.

Reeves may have been an excellent epidemiologist but except for his work with Suzanne Vernon he does not appear to have been a creative thinker. The CDC did lead in some areas. The expensive population studies, for instance, suggested there was little qualitative difference between CFS and CFS-like patients.  No group, however, has shown interest in furthering that line of research.

CDC's research program in the balance

The CDC’s research program has produced positive results.

Reeves singular contribution – the attempt to quantify CFS patients more accurately using SF-36, MFI and symptom scores in the Empirical Definition –  failed to attract interest. Reeve’s development of the ED ultimately isolated the CDC and put it on a confusing course.  Reeves attempt to validate others findings also often ended with sometimes disastrous (orthostatic intolerance) mixed (sleep) or muted results (cognition).

Contrary to popular belief, however, Reeves CDC program did focus heavily on biological issues. Only six of the sixty plus studies Reeves funded focused on psychological or behavioral studies. The psych studies may have gotten the lion’s share of attention but the vast majority of funding at the CDC went to other types of projects – and at times significant progress was made.

Reeves most positive influences may have occurred in three areas – two of which he had little to do with. His epidemiological work helped to validate the high economic costs due to and the low diagnostic rates present in ME/CFS.   The Pharmacogenomics projects birthed the work that Dr. Klimas is doing at NSU, and the Dubbo studies validated and expanded our knowledge of the pathogenic triggers present in ME/CFS.

If the  CDC’s research program has not lived up to its promise there’s no doubt, however, that it has moved our understanding of ME/CFS forward in multiple ways.  If that’s so why is the CDC not just disliked by hated by a portion of the ME/CFS community. Why are it’s accomplishments almost completely unacknowledged?

A Pattern High Profile Failures / Low Profile Successes

I suggest that a pattern of high profile failures and low profile successes have been partly to blame

The successes the CDC has had have not been significant enough to erase the high profile mistakes it's made.

The successes the CDC has had have not been significant enough to erase the high profile mistakes it’s made.

The CDC has been notable in it’s ability to produce high-profile failures that have stuck in the ME/CFS communities mind.  It’s successes, on the hand, have mostly been incremental, tend to involve complex studies that are difficult to understand and explain, and are mostly low profile. It has not produced the kind of breakthrough success that would give the public cause to reassess its opinion.

Studies indicate that negative rather than positive impressions tend to stick in our mind and the CDC has a long history of high profile  negative events which have stuck.

  • The negative stories about the CDC that came out Hilary Johnson’s Osler’s Web stuck. The positive aspects of the CDC’s response to ME/CFS – that fact that it did a 180 degree turn when it appeared the De Freitas findings might turn out –  did not stick.
  • The misappropriation of $13 million from the CDC’s CFS research program in the late 1990’s stuck. Less well-remembered is the CDC’s response: the return of the money plus the first multi-year media campaign ever done by the CDC.
  • The finding that the Empirical Definition increased prevalence rates by 6-10’s and may select depressed patients stuck. Jason’s finding that the ED was not that much less accurate than the CCC in defining new patients correctly did not stick. Nor has the pattern of positive results in pathophysiological studies using the ED been noticed much.
  • The few psychological studies (psychiatric diagnoses, childhood trauma, etc.) Reeves funded really stuck. The many more pathophysiological study Reeves and now Unger have funded haven’t stuck.
  • The problems with the CDC Toolkit stuck. Dr. Unger’s removal of the Toolkit from the site and her efforts using CFSAC members to improve the website have not stuck. The CDC’s extensive use of ME/CFS experts to produce training materials for physicians appears not to have stuck much in the minds of the general ME/CFS community, either.

The Sin of Failure

The CDC has also committed the sin of failure; i.e., the failure to get positive results in areas the ME/CFS community dearly wanted positive results. The chief example is the CDC’s pathogen studies. Between 1992 and 2010 ten CDC studies failed to find any evidence of pathogens in ME/CFS.  Reeves was surely correct to say that the CDC had funded more pathogen studies in ME/CFS than any other group. They also, however, failed to find pathogens more times than any other group.

The CDC, of course, has not been alone in difficulties in finding pathogens. The failure of the huge recent Lipkin/Hornig pathogen and the Blood Safety Research Institute studies demonstrate the difficulty in finding pathogens in this disease. The search is not over; it may be that ME/CFS patients have an atypical form of infection.  Should the CDC have looked for those types of infections? Perhaps, but those kinds of infection are controversial and the decision to stop looking for pathogens was not an unreasonable one.

A Tough Act To Follow

Dr. Unger took over a program with enormous baggage. Reeves is associated with most of the factors the ME/CFS community finds disturbing about the program. Unger, whose work was mostly focused on pathophysiology, was not.

Unger is still using the ED, however; she has not acknowledged that PEM is the core symptom in ME/CFS (but is doing exercise studies), and the CDC’s website — which relies mostly on evidence based studies – still a source of concern to some.

upside to CDC's CFS program

Conclusion: with more upside to the CDC’s CFS program than ever before, it would be a shame to lose it now.

Still, Unger ditched the stress and psychological studies. She began a huge multi-site ME/CFS experts study which could have major ramifications for understanding of how to diagnose ME/CFS, what subsets are present in it and our understanding of disease course, comorbidity and how to treat it. Besides furthering our understanding of the severely ill, the protrayal of the severely ill that will result from the second phase of the study will surely heighten the consciousness about the potential severity of this disease.

Unger’s study slate is entirely pathophysiological. She’s funded gene expression, immune, natural killer cell, gynecological, blood lactate, cognition and telomere studies.  She’s incorporated acute stressors such as exercise into her studies.  She’s communicated with ME/CFS support organizations.  She’s worked with CFSAC members to improve the website. For some in the ME/CFS community, however, it’s still the same CDC: for them few of Unger’s efforts have “stuck”.

This review has covered only the research portion of the CDC. It suggests that while the CDC is not god’s gift to the ME/CFS community neither is it necessarily the bogey man many have made it out to be.

It also suggests that Unger has dramatically changed the CDC’s direction. It suggests  Unger’s program is moving in the right direction. Unger’s outreach to the ME/CFS community, her now strong collaborations with ME/CFS experts, and her pathophysiological focus suggest that the best of the CDC’s CFS research program is ahead of us.  This is not a program we want to lose.

 

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HISTORIC Action to Double NIH Funding For ME/CFS Under Way

Raskin Jamie ME/CFSNOW IS THE TIME

After years of work it’s time to attempt what we’ve never been able to do before – get Congress to force the NIH to double its funding for ME/CFS. Support the historic bill to increase research funding, add new ME/CFS research centers, require the development of a strategic plan, etc.. It will take less than 5 minutes.

Be a part of making history. Find out more about the effort here and join it here. 

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